Composite

Part:BBa_K2335004:Design

Designed by: zhi ling lui   Group: iGEM17_KAIT_JAPAN   (2017-10-25)


GFAPpromoter+HlyD+HlyB+TolC+HlyA+IL-12


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 979
    Illegal NotI site found at 814
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1516
    Illegal BamHI site found at 4339
    Illegal BamHI site found at 5363
    Illegal XhoI site found at 3102
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4451
    Illegal AgeI site found at 3622
    Illegal AgeI site found at 3952
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2040
    Illegal BsaI site found at 5215
    Illegal BsaI.rc site found at 1498
    Illegal BsaI.rc site found at 2413
    Illegal BsaI.rc site found at 2908
    Illegal SapI site found at 2840
    Illegal SapI.rc site found at 2212


Design Notes

Whether membrane protein can be inserted into the membrane of Escherichia coli.


Source

Homo sapiens, Escherichia coli

References